TY - THES T1 - Nanomaterial hurdle technology-based antimicrobial treatment protocol for mung bean sprout production A1 - Vera, Desiree D. A2 - Gabriel, Alonzo A. A2 - Escoval, NiƱaliga H. LA - English PP - Quezon City PB - College of Home Economics, University of the Philippines Diliman YR - 2019 UL - http://www.ds.mainlib.upd.edu.ph/Record/UP-99796217613057576 AB - "This study aimed to identify a suitable decontamination treatment for mung beans artificially inoculated with Salmonella enterica. The fates of S. enterica, in aqueous solutions or artificially inoculated onto mung beans, were determined by soaking in Ag and TiO2 nanomaterial concentrations for 8 h. Results of the study showed that 200 ppm of both Ag and TiO2 nanomaterials reduced the microbial population by 1-2 log CFU/g S. enterica in aqueous solutions. This nanomaterial concentration did not significantly (p<0.05) reduce S. enterica artificially inoculated onto mung beans during an 8-h soaking period compared to the control. To enhance the efficacy of Ag and TiO2 nanomaterials, the selected concentration was combined with acidified (pH = 4.0) or neutral (pH = 7.0) nisin-EDTA solutions (25 mg/kg:0.02 M) and was then tested for antimicrobial efficacy against Salmonella enterica in mung beans during an 8-h soaking period. Acidified nisin-EDTA, alone or in combination with 200 ppm TiO2, resulted to the greatest significant (p<0.05) reduction of about 2 log CFU/g of the microbial population in soaked mung beans among the other tested parameters. Mung beans soaked in these identified treatments were subjected to agitated UV-C exposure for 8 h to further reduce S. enterica population. Salmonella enterica in mung beans showed the greatest population reduction upon 8-h exposure to the treatment: nisin-EDTA (pH 4.0) + TiO2 +UV. However, mung bean sprouts produced from such combination appeared to have inferior qualities compared to those subjected to less severe protocols. Results showed that mung beans and sprouts with superior microbial characteristics, but with inferior quality may be used instead as planting materials and not for consumption. Information generated from this research may be used as baseline empirical data by appropriate agencies in the use / application of nanomaterials in mung bean processing" CN - LG 995 2019 F6 V47 KW - Mung bean. ER -